Light Microscopy

Imaging is a balance between speed and resolution. Different specimens are better suited to different microscopes. Accordingly we provide microscopes to match a wide variety of specimens from whole animals to individual cells, with different resolutions from organs to superresolution as well as different imaging speeds.

Sign up for a microscope introduction before you book one of our microscopes in the booking system Seshat for the first time.

Please contact our staff for more information and help to choose the right equipment.

  • Light sheet microscopy - Zebra fish head
  • Multiphoton microscopy - Pollen
  • Root hair

Zeiss AxioImager - Fluorescence Microscopy

The AxioImager is an upright microscope for fluorescence and transmitted light. It is equipped with filters covering the visible light, 400-720 nm. It has both a color and a monochrome camera for epifluorescent and bright field applications. Tile scanning is available to image large specimens. The Emission filters are suitable for e.g. DAPI, GFP, Cy3, and A647.   

For more information please contact jeremy.adler@igp.uu.se.

Zeiss Light Sheet microscopy

The LightSheet microscope, equipped with 4 lasers (405/488/561/633) is a single plane illumination microscopy that does ultra fast imaging for fluorescently labelled biological samples. Compared to many other imaging techniques LightSheet microscopy allows you to image living samples such as zebra fish and other small embryos over a long time scale without compromising on details. You can discover fluorescently labelled 3D structures in high resolution within your specimen without harming the whole organism. The different ways of mounting and positioning your specimen offers you maximum flexibility of visualizing your sample in all possible angles. LightSheet microscopy allows for fast and sensitive image acquisition. The BioVis LightSheet microscope allows also for imaging of optically cleared samples.

Download the  Light sheet quick guideand the sample preparation to learn more.

For more information please contact matyas.molnar@igp.uu.se or dirk.pacholsky@igp.uu.se.

  • Zebrafish development

  • Blood vessel sprouting

  • Heart beat of Zebrafish

Zeiss LSM700 - confocal microscopy

The Zeiss LSM700 is a state of the art inverted confocal microscope with 4 lasers (405/488/564/633) , 2 fluorescence detectors and one for transmitted light. It provides higher resolution than widefield microscopy. With both oil, water and air objectives it suits different specimens and resolutions. Specimens like cells, zebra fish and organs can be imaged on slides, in petri dishes or on multiwell plates. The motorized stage with X, Y and Z movement, enables 3D datasets, tiling for imaging of large specimens and multiposition time-lapse imaging. The instrument is equipped for Live Cell Imaging, including incubator, temperature control and 5% CO2.

For more information please contact jeremy.adler@igp.uu.se.

Zeiss Elyra S.1 - SIM Super Resolution Microscopy

When ordinary confocal resolution isn’t enough to reveal small structures in the sample, superresolution techniques can help to obtain higher resolution. Superresoulution techniques are breaking the conventional resolution limit for microscopes with special lasers, fluorophores or well-designed optical elements in the microscope lightpath. Structured illumination microscopy (SIM) is a superresolution technique where no special fluorophores and sample preparation is needed. Using a grid pattern illumination, the aperture for the used objective and therefore the resolution can be extended to a factor of 2-2.5.

Download the SIM manual

For more information please contact matyas.molnar@igp.uu.se.  

Zeiss LSM710 NLO - Multiphoton Microscopy

In many cases we need to look deeper in biological samples to see a bigger picture of the specimen and the surrounding biology. Multiphoton microscopy (synonyms: two-photon microscopy, nonlinear microscopy)  is a tool using short-pulsed high power infrared laser for penetrating deeper in biological samples as a normal confocal laser could. This tool can be very useful when deeper 3D imaging in necessary with limited photobleaching. The multiphoton system is a scanning imaging technique; therefore its limitation is speed. The system is operated with the ZEN black software. For 3D rendering, Imaris is available at BioVis.

Download the multiphoton manual and check the NDD filter set. 

For more information please contact matyas.molnar@igp.uu.se.