BioVis

Biological Visualization - Microscopy, Flow Cytometery, Electron Microscopy and Image Analysis

Info

BioVis - Biological Visualisation

The BioVis platform of Uppsala University provides you with a unique combination of technology and know-how for visualization of biological samples at the tissue, cell and subcellular level. We specialize in microscopy, electron microscopy, flow cytometry and image analysis. We can offer state of the art electron- confocal and light microscopy, as well as analytical and imaging flow cytometry and cell sorting.

The Medical & Pharmaceutical faculty at Uppsala University supports BioVis financially, thus the user fees can be kept low. The platform is available for all universities, governmental agencies & private companies. Information about users fees can be found here.

With proper training, provided by us, you can get almost complete access to our equipment.


Entrance Card System

To get access to BioVis premises you need to have a door-card. The new card system does NOT apply for research groups based in Rudbeck Lab, as they have already access. Please contact Jeremy.Adler@igp.uu.se for more details. Observe that your cards will be valid for one year in total.


INFORMATION ABOUT SUMMER CLOSURE 2020

BioVis will close as follows.

Node

Electron Microscopy

Flow Cytometry/Light Microscopy/ Analysis:

July 20 –August 09 (week 30-32)

July 27 – August 09 (week 31-32)

During summer (July/August) we will be low in staff, which will result in less service possible.

Full service in Cell sorting will not be available weeks 31-35.

We also would like to thank Mohan and Sara, researcher at IGP, who will support us in the maintenance of Flow Instruments during summer.

Have a nice summer / Your BioVis team


MAY Newsletter

  • Corona virus precautions
  • BioVis instrument information
  • Webinars on sample preparation and Image Analysis
  • Improve your Imaging
  • New GFP

 CORONA virus precautions

BioVis follows recommendations regarding Corona pandemic.

We are open. But LOW IN STAFF.

In case you are a new user to the instrument – have a colleague with you who can guide and help. Do not start/run projects were intensive help from BioVis staff is needed.

AWECK

  • Avoid touching your eyes/nose/mouth with unwashed hands.
  • Wash your hands frequently & thoroughly, using soap & water for at least 20 seconds. Use alcohol-based hand sanitizer if soap and water aren’t available.
  • Elbow: Cough or sneeze into your flexed elbow a tissue or, then throw the tissue in the trash.
  • Clean and disinfect surfaces and objects that people frequently touch.
  • Keep your distance 1.5m between you and others

                             STAY HOME IN CASE YOU FEEL SICK

Your contacts are

  • FlowCytometry and Light Microscopy :

Dirk Pacholsky and Matyas Molnar

  • Image Analysis and BioVis access cards:

Jeremy Adler

  • Electron Microscopy:

Monika Hodik and Karin Staxäng


BioVis Microscopy: Slide Scanner We are one step closer! The ZEISS Slide Scanner AxioScan is finally installed. We are currently organizing introduction for BioVis staff to be up and running in June. We plan initially to have the instrument on full service – so contact us to get in line. We will not charge our full service fee until we have the instrument implemented into our Instrument introductions. Contact Jeremy Adler for more details.

BioVis Microscopy: STEDYCON Deliverance is delayed due to Corona situation and we expect to have  Abberiors STEDYCON up&running after summer.

BioVis Electron microscopy: The EM node would like to promote its available services:  sample preparation assistance for ultrastructural analysis, immunogold labelling and negative staining as well as imaging of ultrathin sections. We have expertise in many different samples, ranging from tissues to biomolecules. For those who wish, we can train you to take images by yourself at the microscope -no, it is not that difficult.  If you have any suggestions of improvements/new techniques that you wish us to implement in our service, (e.g. correlative light and electron microscopy, STEM etc.), we would be happy if you shared it with us.

BioVis Flow Cytometry: A few news here combined. First and foremost: We have to postpone the planned seminar on  tSNE (May 28/29) into the future, which might be autumn 2020. We are currently planning a  substitute webinar together with BD in using FlowJo – also here in respect  to tSNE analysis. To accompany that with the hardware, i.e. Flow Cytometer, we are also planning to upgrade the current optical configurations on the BD instrument we own -  to reach more fluorescent parameter. In case you are having a hard time to find a good panel for Multicolor Immunofluorescence: A total of 62 Optimized Multicolor Immunofluorescence Panel (OMPIs) panels are currently published. Those OMIP's are not company-based but researchers' panels and peer-reviewed


Webinar: Advanced optical microscopy sample preparation

The ALM Facility cordially welcomes you to a webinar on June 10, covering advanced optical microscopy sample preparation applications from world-leading experts. The program covers Electron Microscopy, and Super Resolution in respect to Live Cell imaging and expansion microscopy DATE: June 10th 9-11am.


Webinar: Image analysis on NEUBIAS

A plethora of fantastic webinar and events in regard to image analysis can be found (and applied for) on NEUBIAS homepage. This initiative aims to provide material and activities focused on Bioimage Analysis training. Events are also recorded and available on their youtube channel.


Webinar: Image analysis on iBiology

Another haven of Knowledge is iBiology, a channel on Youtube which brings to you amongst other things lecture series about imaging techniques like Confocal, STED, … as well Image Analysis.


Improving your confocal microscopy

Confocal microscopes have many settings; pixel size, pixel number, pinhole size, resolution, scanning options, wavelength ranges etc. A recent review “Tutorial: guidance for quantitative confocal microscopy” by Jonkman et al., describes what each means, the balance between each and how they contribute to producing images that answer your biological question. Confocal microscopes are inherently slow, so making the best choices employs your time efficiently.Feel to download the accompanying poster.


New Fluorescent Proteins – replacing EGFP

The EGFPs and variants are the most common fluorescent tags for expressed proteins but new and better FPs are emerging. AausFP1 green fluorescent protein 5x brighter than EGFP, is not excitable with at 405nm, has a narrower emission range and faster maturation – all positive features. It also has a near perfect quantum efficiency and efficiently captures excitation photons. Variants with longer emission ranges where also found along with photoconvertible FPs – these can be activated at specified locations  and then followed in vivo.


Have a sunny MAY and stay healthy

Your BioVis team

 


You can find us

Uppsala , Science Park Rudbeck Laboratory, 2nd floor

Dag Hammarskjölds v 20
751 85 UPPSALA, SWEDEN