BioVis

Biological Visualization - Microscopy, Flow Cytometery, Electron Microscopy and Image Analysis

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BioVis - Biological Visualisation

The BioVis platform of Uppsala University provides you with a unique combination of technology and know-how for visualization of biological samples at the tissue, cell and subcellular level. We specialize in Light- and, Electron-Microscopy, Flow Cytometry and Data analysis. We can offer state of the art Electron-Microscopy and sample preparation,  Confocal- and Superresolution-Microscopy, 2Photon- and Slide Scanning Microscopy, as well as analytical Flow Cytometry and Cell Sorting. See more information here. BioVis is hosted by the IGP department and situated in the Rudbeck Laboratory, Science Park, Uppsala.The Medical & Pharmaceutical faculty at Uppsala University supports BioVis financially, thus the user fees can be kept low. The platform is available for all universities, governmental agencies & private companies. Information about users fees can be found here.With proper training, provided by us, you can get almost complete access to our equipment.

Head of BioVis Dirk.Pacholsky@igp.uu.se


BioVis Newsletter August 2022

Workshop, Symposia & Demo

  • WORKSHOP: ZEISS Slide Scanner, get a more detailed insight in how to use the instrument. August 26th, Friday, lecture and workshop.The lecture will be given 10:15 12:00, Waldenström room, Rudbeck Lab. Contact Jeremy Adler to register.

  • NIKON DEMO and SYMPOSIUM

Get a view on two new instruments from Nikon: AX/AX R – Nikon’s 10th generation point scanning confocal system (Nikon) and Nikon/CrEST V3-DeepSIM – a large field-of-view spinning disk confocal system combined with structured illumination super-resolution capabilities (CrestOptics). August 31 – September 16. In conjunction with NIKON´s Instrument demo a Symposium will be given September 13 (10:00-13:00).

Schedule

10:00-10:05 Welcome, Oliver Garner, BergmanLabora AB

10:05-10:50 Photodamage in sensitive samples (live or dim) – dos and don’ts, Sylvie Le Guyader – Facility Manager, Live Cell Imaging Facility /Nikon Center of Excellence, Karolinska Institute

10:50-11:30 Advances in Confocal and Super-Resolution microscopy, Oliver Garner, BergmanLabora AB

11:30-12:15 Data-driven image acquisition to enable true quantitative microscopy, Pontus Nordenfelt – Associate Professor, Infection Medicine, Lund University

12:15-13:00 Lunch

13:00-16:00 Open-house for demonstration systems. Nikon AX R confocal and Nikon/CrEST V3 spinning disk with DeepSIM super-resolution


Registration for both events  is free, but mandatory Latest Friday 9th September 2022. Registration and  more Information on the Event site.

  • SUPERRESOLUTION SYMPOSIUM, September 28th, organized by Hans Blom of the Advanced Light Microscopy (ALM) Facility, Stockholm LINK, project discussions with the experts possible

FlowCytometry

  • BioVis  will install a new Flow Cytometer week 34:  the BeckmanCoulter Cytoflex LX (link)with 6 Laser and  21 FL channel The instrument has the Laser UV/405/488/561/638/808 and  plateloader for a standard and deep well 96 plates , beside its regular tube loader. The detector  configuration can be UV3-V5-B3-Y5-R3-I2 or UV7-V5-B3-Y5-R3-I0 and offers otherwise the flexibility we know from our Cytoflex S. Switching between the S and LX will be a breeze.

Microscopy

  • STED, see more details when needed: Resolution down to 40nm (XY) by using our STEDYCON microscope. Let´s get you jumpstarted with secondary Abs, ready for STED - discuss you project with Matyas.
  • Deconvolution, see more details by deconvolving your images from Confocal and Widefield microscopy. Let´s get you jumpstarted - discuss you project with Jeremy.
  • Our Leica DIVE SP8 2PhotonMicroscope is up and running, perfect for InVitro Imaging and Deep tissue Imaging. Objectives for Optically Cleared Samples are in place. Contact Matyas Molnar for more details.
  • Thanks to a donation from Lena Claesson Welsh we do have now 2 Confocal LSM700. Available for booking. Contact Jeremy for a brief introduction.

Courses

  • Applications to our PhD courses “Methods for Cell Analysis” (October) and “Introductions to Image Analysis software's” (September) are open, as well for our BioVis courses regarding Image J. Follow this LINK. The IAS course (Sept01-07, 1.5hp) has still 2 places available. Contact Jeremy Adler for registration

Sellout

  • BioVis is selling its ZEISS Lightsheet Z1, ready for living and optically cleared  samples. See more details in the document here.
  • SIM discontinuation:Our Zeiss LSM710 SIM (Elyra S.1) will be discontinued in June 2022. Alternatives for higher resolution in Biovis are confocal imaging with deconvolution; or STED superresolution. Interested? Make us an offer.

more interesting News:

  • Join the Confocal user group to discuss microscopes and  imaging
  • Join the Flow Cytometry User group to discuss Flow Cytometry
  • A New Spectra viewer is online with over 550 fluorophores, containing also 2Photon excitation, uploading of custom spectra possibilities, 2D contour plots and accessibility via mobile phones.
  • A New Facility for Single Particle Analysis using Electronmicroscopy: The new Glacios™ Cryo-TEM for single particle analysis was inaugurated on the 3rd of March at ICM/BMC. For those interested in using the instrument in their research, please contact Anna Sundborger-Lunna or Daniel Larsson (cryoem-icm@uu.se) for further information. For those interested in performing negative staining of their samples prior cryo-EM, please contact Monika Hodik (Monika.Hodik@igp.uu.se) or Karin Staxäng (Karin.Staxang@igp.uu.se) at the BioVis EM node.


Biological Visualisation

The BioVis platform provides you with a unique combination of technology and know-how for visualization of biological samples at the tissue, cell and subcellular level. We specialise in microscopy, electron microscopy, flow cytometry and image analysis. We can offer state of the art electron- confocal and light microscopy, as well as analytical and imaging flow cytometry and cell sorting.

The Medical and Pharmaceutical faculty at Uppsala University supports BioVis financially, thus the user fees can be kept low. The platform is available for all universities, governmental agencies and private companies. With proper training, provided by us, you can get almost complete access to the equipment at BioVis.

You can find us

Uppsala , Science Park Rudbeck Laboratory, 2nd floor

Dag Hammarskjölds v 20
751 85 UPPSALA, SWEDEN

Last modified: 2022-08-22