BioVis

Biological Visualization - Microscopy, Flow Cytometery, Electron Microscopy and Image Analysis

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BioVis - Biological Visualisation

The BioVis platform of Uppsala University provides you with a unique combination of technology and know-how for visualization of biological samples at the tissue, cell and subcellular level. We specialize in Light- and, Electron-Microscopy, Flow Cytometry and Data analysis. We can offer state of the art Electron-Microscopy and sample preparation,  Confocal- and Superresolution-Microscopy, 2Photon- and Slide Scanning Microscopy, as well as analytical Flow Cytometry and Cell Sorting. See more information here. BioVis is hosted by the IGP department and situated in the Rudbeck Laboratory, Science Park, Uppsala.The Medical & Pharmaceutical faculty at Uppsala University supports BioVis financially, thus the user fees can be kept low. The platform is available for all universities, governmental agencies & private companies. Information about users fees can be found here.With proper training, provided by us, you can get almost complete access to our equipment.

Head of BioVis Dirk.Pacholsky@igp.uu.se


BioVis Newsletter May 2022

SummerClosure

  • For all you who have to plan – BioVis will close
  • FlowCytometry and  Microscopy:                            July 02 – July 17
  • ElectronMicroscopy:                                                 July 18 – August 07
  • Week 29  and 30 BioVis Microscopy and Flow Cytometry is undermanned and the following instruments are not available: STED, 2PM.

FlowCytometry

  • BioVis  will install around June a new Flow Cytometer:  the BeckmanCoulter Cytoflex LX (link)with 6 Laser and  21 FL channel The instrument has the Laser UV/405/488/561/638/808 and  plateloader for a standard and deep well 96 plates , beside its regular tube loader. The detector  configuration can be UV3-V5-B3-Y5-R3-I2 or UV7-V5-B3-Y5-R3-I0 and offers otherwise the flexibility we know from our Cytoflex S. Switching between the S and LX will be a breeze.

Microcopy

  • DEMO: Have a look at the “jack of all trades” microscope – The Andor BC-43 (link) a combination of widefield, spinning disk confocal  & live imaging. Speed and ease of use are the attractions. There will be a presentation on the Monday 30th May and a chance to test the microscope with your specimens on Tuesday 31st If interested, contact: jeremy.adler@igp.uu.se
  • DEMO:Have a look at  Nikon´s Confocal (link) around week 34 or 35, more info will come soon.
  • The AxioImager is now placed in the FlowCytometry room and up and running. (not networked yet).
  • STED, see more details when needed: Resolution down to 40nm (XY) by using our STEDYCON microscope. Let´s get you jumpstarted with secondary Abs, ready for STED - discuss you project with Matyas.
  • Deconvolution, see more details by deconvolving your images from Confocal and Widefield microscopy. Let´s get you jumpstarted - discuss you project with Jeremy.

Courses

  • Applications to our PhD courses “Methods for Cell Analysis” (October) and “Introductions to Image Analysis software's” (June, September) are open, as well for our BioVis courses regarding Image J. Follow this LINK.

Fluorophores

  • The regular DAPI is  ´barely´excited by the 405 Laser usually used. Get better Nucleus images on Confocal Microscopes – replace DAPI with Hoechst43580 or Nuclear Violet. Cell permeable and hence applicable for Live Cell Imaging.

Sellout

  • BioVis is selling its ZEISS Lightsheet Z1, ready for living and optically cleared  samples. See more details in the document here.
  • SIM discontinuation:Our Zeiss LSM710 SIM (Elyra S.1) will be discontinued in June 2022. Alternatives for higher resolution in Biovis are confocal imaging with deconvolution; or STED superresolution

more interesting News:

  • Join the Confocal user group to discuss microscopes and  imaging
  • Join the Flow Cytometry User group to discuss Flow Cytometry
  • A New Spectra viewer is online with over 550 fluorophores, containing also 2Photon excitation, uploading of custom spectra possibilities, 2D contour plots and accessibility via mobile phones.
  • A New Facility for Single Particle Analysis using Electronmicroscopy: The new Glacios™ Cryo-TEM for single particle analysis was inaugurated on the 3rd of March at ICM/BMC. For those interested in using the instrument in their research, please contact Anna Sundborger-Lunna or Daniel Larsson (cryoem-icm@uu.se) for further information. For those interested in performing negative staining of their samples prior cryo-EM, please contact Monika Hodik (Monika.Hodik@igp.uu.se) or Karin Staxäng (Karin.Staxang@igp.uu.se) at the BioVis EM node.

Biological Visualisation

The BioVis platform provides you with a unique combination of technology and know-how for visualization of biological samples at the tissue, cell and subcellular level. We specialise in microscopy, electron microscopy, flow cytometry and image analysis. We can offer state of the art electron- confocal and light microscopy, as well as analytical and imaging flow cytometry and cell sorting.

The Medical and Pharmaceutical faculty at Uppsala University supports BioVis financially, thus the user fees can be kept low. The platform is available for all universities, governmental agencies and private companies. With proper training, provided by us, you can get almost complete access to the equipment at BioVis.

You can find us

Uppsala , Science Park Rudbeck Laboratory, 2nd floor

Dag Hammarskjölds v 20
751 85 UPPSALA, SWEDEN

Last modified: 2022-05-13